A second overseas charter flight of players and participants arriving in Melbourne for the Australian Open has recorded a positive COVID-19 case.

A passenger onboard a charter flight that arrived in Melbourne at 8.20am on January 15 from Abu Dhabi has tested positive.

The passenger is not a player and has been transferred to a health hotel to undertake a 14-day quarantine period. The passenger tested negative to their pre-departure test.

A bus transports Australian Open players and officials to the Grand Hyatt in Melbourne, where they will undergo 14 days in quarantine. (9News)Tennis players carrying tennis bags are seen disembarking Flight EY460 carrying tennis players and their support teams participating at the Australian Open at Melbourne Jet Base adjoining the Melbourne International airport on January 14, 2021 in Melbourne, Australia. (Getty)

It’s the third positive case found across two charter flights of participants and players arriving in Australia for the Australian Open.

A spokesperson for COVID-19 Quarantine Victoria said all remaining 63 passengers on the plane – which include players – must also undertake two weeks in quarantine and will be unable to train.

“All remaining 63 passengers on the flight have been designated close contacts. Players and support people will be unable to access training and must undertake a standard 14-day quarantine period.

World Number 2 player Rafael Nadal has arrived in Australia and entered hotel quarantine, ahead of the Australian Open on February 8. (9News)

“Players are being supported to access equipment for their hotel rooms to help them maintain their fitness during this time.

“There are no other known positive tests from this flight, but routine testing will continue for passengers.

“Before any person can exit quarantine after 14 days, they must be first medically cleared by public health experts.”

In its second statement for the night, Australian Open organisers confirmed that 23 of the passengers onboard the second flight were passengers.

All of the passengers are already in quarantine hotels and the positive case is in a specially-designed “health hotel” for positive cases.

In an email to players, which has since been shared online, health authorities assured players that they are doing everything they can to “mitigate the impact” of being forced to spend 14 days in a hotel room prior to a tournament.

“We are aware of the major impact this has on your preparation for the Australian summer,” the email reads.

“And are going to do everything we can to mitigate this impact. Our entire team is mobilised and here to support you.

“We will do everything that we can to get you through this.”

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